Proteintech, 84329-1-RR, Di/Tri-Methyl-Histone H3 (Lys36) Recombinant monoclonal antibody

Size: 20ul / 100ul The Di/Tri-Methyl-Histone H3 (Lys36) (84329-1-RR) by Proteintech is a Recombinant antibody targeting Di/Tri-Methyl-Histone H3 (Lys36) in WB, IHC, IF/ICC, Dot Blot, ELISA applications with reactivity to human, mouse, rat samples 84329-1-RR targets Di/Tri-Methyl-Histone H3 (Lys36) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, U-87 MG cells, U-251 cells, NIH/3T3 cells, mouse liver tissue, mouse brain tissue, HSC-T6 cells Positive IHC detected in: human placenta tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive Dot Blot detected in: peptide Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 DOT BLOT: DOT BLOT : 1:10-1:100 Background Information Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 15 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 RRID: AB_3671870 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.