Proteintech, 84369-5-RR, CD44 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD44 (84369-5-RR) by Proteintech is a Recombinant antibody targeting CD44 in WB, IHC, IF/ICC, ELISA applications with reactivity to rat samples 84369-5-RR targets CD44 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with rat samples. Tested Applications Positive WB detected in: rat spleen tissue Positive IHC detected in: rat spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: NR8383 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Specification Tested Reactivity: rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: Cd44 molecule Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 80 kDa GenBank Accession Number: XM_006234631.3 Gene Symbol: Cd44 Gene ID (NCBI): 25406 RRID: AB_3671905 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O70509 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.