Proteintech, 84378-5-RR, CYP2R1 Recombinant monoclonal antibody

Size: 20ul / 100ul The CYP2R1 (84378-5-RR) by Proteintech is a Recombinant antibody targeting CYP2R1 in WB, FC (Intra), ELISA applications with reactivity to human samples 84378-5-RR targets CYP2R1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, HuH-7 cells, human placenta tissue Positive FC (Intra) detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CYP2R1 is a member of the cytochrome P450 superfamily of enzymes which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. CYP2R1 primarily functions as a 25-hydroxylase of vitamin D, responsible for converting vitamin D to 25-hydroxyvitamin D (25-OH-D), affecting the production of the active hormone 1,25(OH)2D (PMID: 12867411). CYP2R1 enzyme is a key vitamin D 25-hydroxylase, mutations in CYP2R1 are associated with Rickets vitamin D-dependent 1B(PMID: 25942481, 15128933). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag12873 Product name: Recombinant human CYP2R1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 165-501 aa of BC104909 Sequence: FNDAIETYKGRPFDFKQLITNAVSNITNLIIFGERFTYEDTDFQHMIELFSENVELAASASVFLYNAFPWIGILPFGKHQQLFRNAAVVYDFLSRLIEKASVNRKPQLPQHFVDAYLDEMDQGKNDPSSTFSKENLIFSVGELIIAGTETTTNVLRWAILFMALYPNIQGQVQKEIDLIMGPNGKPSWDDKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDAVVRGYSIPKGTTVITNLYSVHFDEKYWRDPEVFHPERFLDSSGYFAKKEALVPFSLGRRHCLGEHLARMEMFLFFTALLQRFHLHFPHELVPDLKPRLGMTLQPQPYLICAERR Predict reactive species Full Name: cytochrome P450, family 2, subfamily R, polypeptide 1 Calculated Molecular Weight: 501 aa, 57 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: BC104909 Gene Symbol: CYP2R1 Gene ID (NCBI): 120227 RRID: AB_3671915 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6VVX0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.