Proteintech, 84387-6-RR, CD98/SLC3A2 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD98/SLC3A2 (84387-6-RR) by Proteintech is a Recombinant antibody targeting CD98/SLC3A2 in WB, IF/ICC, ELISA applications with reactivity to human samples 84387-6-RR targets CD98/SLC3A2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, MCF-7 cells, HepG2 cells, Raji cells, T-47D cells Positive IF/ICC detected in: HepG2 cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter) and an 85-kDa glycosylated heavy chain (CD98hc) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg1948 Product name: Recombinant Human CD98/SLC3A2 protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 206-630 aa of NM_002394.5 Sequence: RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA Predict reactive species Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 68-125 kDa GenBank Accession Number: NM_002394.5 Gene Symbol: CD98 Gene ID (NCBI): 6520 RRID: AB_3671923 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P08195-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.