Proteintech, 84434-4-RR, CD73 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD73 (84434-4-RR) by Proteintech is a Recombinant antibody targeting CD73 in WB, IF/ICC, ELISA applications with reactivity to mouse samples 84434-4-RR targets CD73 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IF/ICC detected in: 4T1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information CD73, also known as ecto-5'-nucleotidase (5'-NT), is a glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: FusionProtein Predict reactive species Full Name: 5' nucleotidase, ecto Calculated Molecular Weight: 64kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: NM_011851.4 Gene Symbol: Nt5e Gene ID (NCBI): 23959 RRID: AB_3671960 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q61503 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.