Proteintech, 84474-3-RR, CD107b / LAMP2 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD107b / LAMP2 (84474-3-RR) by Proteintech is a Recombinant antibody targeting CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications with reactivity to mouse samples 84474-3-RR targets CD107b / LAMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, mouse liver tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides. Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: lysosomal-associated membrane protein 2 Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: NM_001017959.2 Gene Symbol: CD107b / LAMP2 Gene ID (NCBI): 16784 RRID: AB_3671996 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P17047-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.