Proteintech, 84519-4-RR, CD55 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD55 (84519-4-RR) by Proteintech is a Recombinant antibody targeting CD55 in WB, ELISA applications with reactivity to mouse samples 84519-4-RR targets CD55 in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse testis tissue, mouse spleen tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: FusionProtein Predict reactive species Full Name: CD55 antigen Calculated Molecular Weight: 43kDa Observed Molecular Weight: 58-70 kDa GenBank Accession Number: NM_010016.3 Gene Symbol: CD55 Gene ID (NCBI): 13136 RRID: AB_3672026 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q61475 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.