Proteintech, 84551-1-RR, Acetyl-Histone H2B (Lys120) Recombinant monoclonal antibody

Size: 20ul / 100ul The Acetyl-Histone H2B (Lys120) (84551-1-RR) by Proteintech is a Recombinant antibody targeting Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 84551-1-RR targets Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Trichostatin A treated NIH/3T3 cells Positive IF/ICC detected in: Trichostatin A treated HeLa cells Positive Dot Blot detected in: peptide Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 2, H2be Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC005827 Gene Symbol: Histone H2B Gene ID (NCBI): 8349 RRID: AB_3672059 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q16778 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.