Proteintech, 84563-1-RR, Phospho-SH3PXD2B (Ser291) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-SH3PXD2B (Ser291) (84563-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-SH3PXD2B (Ser291) in WB, IF/ICC, ELISA applications with reactivity to human samples 84563-1-RR targets Phospho-SH3PXD2B (Ser291) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells Positive IF/ICC detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SH3 and PX domains 2B (SH3PXD2B) is also known as tyrosine kinase substrate with four SH3 domains (Tks4). It consists of the N-terminal phox homology domain, four Sr homology 3 domains, several proline-rich regions, and Src phosphorylation sites. Previous studies have shown that SH3PXD2B is an essential invadopodium scaffold protein and can stimulate actin polymerization, recruit membrane type-1 matrix metalloproteinase (MT1-MMP) to intracellular membranes, and increase Nox1-dependent reactive oxygen species (ROS) production. (PMID: 33906640) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SH3 and PX domains 2B Calculated Molecular Weight: 911aa, 102 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: NM_001017995 Gene Symbol: SH3PXD2B Gene ID (NCBI): 285590 RRID: AB_3672071 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: A1X283 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.