Proteintech, 84580-4-RR, CD99 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD99 (84580-4-RR) by Proteintech is a Recombinant antibody targeting CD99 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 84580-4-RR targets CD99 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: THP-1 cells, Jurkat cells, HUVEC cells, U-937 cells, U-251 cells, MOLT-4 cells, human testis tissue Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: CD99 molecule Calculated Molecular Weight: 19 kDa, 16 kDa, 17 kDa Observed Molecular Weight: 28 kDa, 32 kDa GenBank Accession Number: BC021620 Gene Symbol: CD99 Gene ID (NCBI): 4267 RRID: AB_3672079 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P14209 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.