Proteintech, 84588-1-RR, MAN1B1 Recombinant monoclonal antibody

Size: 20ul / 100ul The MAN1B1 (84588-1-RR) by Proteintech is a Recombinant antibody targeting MAN1B1 in WB, FC (Intra), ELISA applications with reactivity to human samples 84588-1-RR targets MAN1B1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, HuH-7 cells, HeLa cells, LNCaP cells, HEK-293 cells, U-87 MG cells Positive FC (Intra) detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag28302 Product name: Recombinant human MAN1B1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 350-699 aa of BC002953 Sequence: LFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA Predict reactive species Full Name: mannosidase, alpha, class 1B, member 1 Calculated Molecular Weight: 80 kDa Observed Molecular Weight: 80 kDa GenBank Accession Number: BC002953 Gene Symbol: MAN1B1 Gene ID (NCBI): 11253 RRID: AB_3672084 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9UKM7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.