Proteintech, 84606-6-RR, DPP4/CD26 Recombinant monoclonal antibody

Size: 20ul / 100ul The DPP4/CD26 (84606-6-RR) by Proteintech is a Recombinant antibody targeting DPP4/CD26 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 84606-6-RR targets DPP4/CD26 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells, human placenta tissue, LNCaP cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HuH-7 cells, PC-3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg0157 Product name: Recombinant Human DPP4/CD26 protein (Myc Tag, His Tag) Source: mammalian cells -derived, pHZ-KIsec Tag: Myc & 6*His Domain: 34-766 aa of BC013329 Sequence: DATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP Predict reactive species Full Name: dipeptidyl-peptidase 4 Calculated Molecular Weight: 88 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC013329 Gene Symbol: DPP4 Gene ID (NCBI): 1803 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P27487 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.