Proteintech, 84654-4-RR, NEK4 Recombinant monoclonal antibody

Size: 20ul / 100ul The NEK4 (84654-4-RR) by Proteintech is a Recombinant antibody targeting NEK4 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 84654-4-RR targets NEK4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, HEK-293T cells, HeLa cells, NCI-H1299 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:400-1:1600 Background Information Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34929 Product name: Recombinant human NEK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 429-779 aa of BC063044 Sequence: SSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYR Predict reactive species Full Name: NIMA (never in mitosis gene a)-related kinase 4 Calculated Molecular Weight: 781 aa, 88 kDa Observed Molecular Weight: 95-100 kDa GenBank Accession Number: BC063044 Gene Symbol: NEK4 Gene ID (NCBI): 6787 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P51957 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.