Proteintech, 84727-5-RR, TMIGD2/CD28H Recombinant monoclonal antibody

Size: 20ul / 100ul The TMIGD2/CD28H (84727-5-RR) by Proteintech is a Recombinant antibody targeting TMIGD2/CD28H in WB, ELISA applications with reactivity to human, pig samples 84727-5-RR targets TMIGD2/CD28H in WB, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: Jurkat cells, pig thymus tissue, MOLT-4 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704). The predicted molecular weight of the TMIGD2 protein is 31 kDa. The higher molecular weight of TMIGD2 could be associated with its potential glycosylation at its extracellular region (PMID: 22419821). Specification Tested Reactivity: human, pig Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: transmembrane and immunoglobulin domain containing 2 Calculated Molecular Weight: 282 aa, 31 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC015655 Gene Symbol: TMIGD2 Gene ID (NCBI): 126259 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q96BF3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.