Proteintech, 84739-2-RR, Phospho-Histone H3 (Thr3) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Histone H3 (Thr3) (84739-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-Histone H3 (Thr3) in WB, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 84739-2-RR targets Phospho-Histone H3 (Thr3) in WB, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Calyculin A treated HeLa cells Positive FC (Intra) detected in: Calyculin A treated NIH/3T3 cells Positive ChIP-qPCR detected in: Nocodazole (100 ng/ml, 16 h) HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 17 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 RRID: AB_3672124 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.