Proteintech, 84803-4-RR, ZDHHC5 Recombinant monoclonal antibody

Size: 20ul / 100ul The ZDHHC5 (84803-4-RR) by Proteintech is a Recombinant antibody targeting ZDHHC5 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 84803-4-RR targets ZDHHC5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, A549 cells, mouse brain tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag15022 Product name: Recombinant human ZDHHC5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 313-662 aa of BC026967 Sequence: AMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSEPSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPRLVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLGKTPLGRPAVPRFGKPDGLRGRGVGSPEPGPTAPYLGRSMSYSSQKAQPGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSLGSASPGPGQPPLSSPTRGGVKKVSGVGGTTYEISV Predict reactive species Full Name: zinc finger, DHHC-type containing 5 Calculated Molecular Weight: 715 aa, 78 kDa Observed Molecular Weight: 75-77 kDa GenBank Accession Number: BC026967 Gene Symbol: ZDHHC5 Gene ID (NCBI): 25921 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9C0B5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.