Proteintech, 84817-4-RR, CD229/SLAMF3 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD229/SLAMF3 (84817-4-RR) by Proteintech is a Recombinant antibody targeting CD229/SLAMF3 in WB, IHC, ELISA applications with reactivity to mouse samples 84817-4-RR targets CD229/SLAMF3 in WB, IHC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: EL-4 cells, mouse thymus tissue, mouse spleen tissue Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Signaling lymphocytic activation molecule 3 (SLAMF3), also known as CD229 or Ly9, is a type I transmembrane glycoprotein that belongs to the SLAM family receptors (PMID: 10970093; 15905546). It is composed of an extracellular region, which consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail, which includes two immunoreceptor tyrosine-based signaling motifs (ITSMs) and other tyrosine residues that act as binding sites for the SH2 structural domains (PMID: 8537117; 11369645; 38626547). SLAMF3 is mainly expressed in immune cells, such as T, B, and natural killer cells, and it is involved in the fundamental activation and suppression of these immune cells, as well as the regulation of complex immune networks (PMID: 38626547). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: lymphocyte antigen 9 Calculated Molecular Weight: 73 kDa Observed Molecular Weight: 80-90 kDa, 110 kDa GenBank Accession Number: NM_001277968.1 Gene Symbol: Ly9 Gene ID (NCBI): 17085 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q01965 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.