Proteintech, 84839-1-RR, Phospho-EGFR (Tyr869) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-EGFR (Tyr869) (84839-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-EGFR (Tyr869) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 84839-1-RR targets Phospho-EGFR (Tyr869) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: EGF treated A431 cells Positive IF/ICC detected in: EGF treated A431 cells Positive FC (Intra) detected in: EGF treated A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. After EGFR homodimerization with extracellular ligands such as epidermal growth factor (EGF), the intracellular tyrosine kinase domain is activated and autophosphorylates multiple tyrosine residues in the C‐terminal tail domain. (PMID: 23822636) Some scientists call EGFR Tyr869 as EGFR Tyr845, the site where the signaling peptide is removed. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Calculated Molecular Weight: 1210 aa, 134 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: BC094761 Gene Symbol: EGFR Gene ID (NCBI): 1956 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P00533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.