Proteintech, 84854-5-RR, CD44 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD44 (84854-5-RR) by Proteintech is a Recombinant antibody targeting CD44 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 84854-5-RR targets CD44 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MDA-MB-231 cells, A549 cells, PC-3 cells, PANC-1 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: CD44 molecule (Indian blood group) Calculated Molecular Weight: 82 kDa Observed Molecular Weight: 80-95 kDa GenBank Accession Number: NM_000610.3 Gene Symbol: CD44 Gene ID (NCBI): 960 ENSEMBL Gene ID: ENSG00000026508 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P16070-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.