Proteintech, 84908-2-RR, Di/Tri-Methyl-Histone H3 (Lys4) Recombinant monoclonal antibody

Size: 20ul / 100ul The Di/Tri-Methyl-Histone H3 (Lys4) (84908-2-RR) by Proteintech is a Recombinant antibody targeting Di/Tri-Methyl-Histone H3 (Lys4) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse, rat samples 84908-2-RR targets Di/Tri-Methyl-Histone H3 (Lys4) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells Positive IHC detected in: mouse testis tissue, mouse liver tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive Dot Blot detected in: peptide Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.