Proteintech, 84911-3-RR, CCR2a-specific Recombinant monoclonal antibody

Size: 20ul / 100ul The CCR2a-specific (84911-3-RR) by Proteintech is a Recombinant antibody targeting CCR2a-specific in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 84911-3-RR targets CCR2a-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: U-937 cells, THP-1 cells, A431 cells, Jurkat cells Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:10000-1:40000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: chemokine (C-C motif) receptor 2 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: NM_000647 Gene Symbol: CCR2 Gene ID (NCBI): 729230 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P41597 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.