Proteintech, 84953-4-RR, Cystatin A Recombinant monoclonal antibody

Size: 20ul / 100ul The Cystatin A (84953-4-RR) by Proteintech is a Recombinant antibody targeting Cystatin A in WB, IHC, ELISA applications with reactivity to human samples 84953-4-RR targets Cystatin A in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U2OS cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information Cystatin A (CSTA) is a member of the cystatin superfamily, which are thiol proteinase inhibitors (TPI) that play crucial roles in regulating proteolytic activity. It is a small protein composed of 98 amino acids with a molecular weight of approximately 11 kDa. CSTA is primarily expressed in epithelial tissues, such as the skin and esophagus, where it serves as a precursor protein for the cornified cell envelope in keratinocytes. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8733 Product name: Recombinant human CSTA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-98 aa of BC010379 Sequence: MIPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLVLTGYQVDKNKDDELTGF Predict reactive species Full Name: cystatin A (stefin A) Calculated Molecular Weight: 98 aa, 11 kDa Observed Molecular Weight: 11 kDa GenBank Accession Number: BC010379 Gene Symbol: Cystatin A Gene ID (NCBI): 1475 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P01040 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.