Proteintech, 84980-1-RR, Cyclin T1 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cyclin T1 (84980-1-RR) by Proteintech is a Recombinant antibody targeting Cyclin T1 in WB, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples 84980-1-RR targets Cyclin T1 in WB, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells Positive FC (Intra) detected in: MCF-7 cells, U2OS cells Positive ChIP-qPCR detected in: HCT 116 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information CCNT1, also named as Cyclin T1 and CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cyclin T1 Calculated Molecular Weight: 81 kDa Observed Molecular Weight: 75-81 kDa GenBank Accession Number: NM_001240 Gene Symbol: Cyclin T1 Gene ID (NCBI): 904 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O60563 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.