Proteintech, 85078-5-RR, HNRNPR Recombinant monoclonal antibody

Size: 20ul / 100ul The HNRNPR (85078-5-RR) by Proteintech is a Recombinant antibody targeting HNRNPR in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 85078-5-RR targets HNRNPR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse brain tissue, HepG2 cells, HEK-293T cells, Jurkat cells, U2OS cells, rat brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag30339 Product name: Recombinant human HNRNPR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 423-475 aa of BC001449 Sequence: QAARQASRSTAYEDYYYHPPPRMPPPIRGRGRGGGRGGYGYPPDYYGYEDYYD Predict reactive species Full Name: heterogeneous nuclear ribonucleoprotein R Calculated Molecular Weight: 71 kDa Observed Molecular Weight: 65-82 kDa GenBank Accession Number: BC001449 Gene Symbol: HNRNPR Gene ID (NCBI): 10236 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43390 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.