Proteintech, 85139-9-RR, EPHB4 Recombinant monoclonal antibody

Size: 20ul / 100ul The EPHB4 (85139-9-RR) by Proteintech is a Recombinant antibody targeting EPHB4 in WB, ELISA applications with reactivity to human samples 85139-9-RR targets EPHB4 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells, HeLa cells, MCF-7 cells, HT-29 cells, HT-1080 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information EPHB4 (Ephrin type-B receptor 4) is a member of the Eph receptor tyrosine kinase family, the largest group of receptor tyrosine kinases. It binds ephrin-B ligands to regulate embryonic vascular development, lymphangiogenesis, and adult angiogenesis. Aberrant EPHB4 signaling promotes tumor progression, metastasis, and therapy resistance in various cancers, including breast, prostate, and colorectal malignancies. As a key regulator of tumor vasculature and the tumor microenvironment, EPHB4 represents an emerging therapeutic target for anti-cancer drug development and a potential prognostic biomarker. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg6504 Product name: Recombinant Human EPHB4 protein (rFc Tag) Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 16-539 aa of NM_004444.4 Sequence: LEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLA Predict reactive species Full Name: EPH receptor B4 Calculated Molecular Weight: 108 kDa Observed Molecular Weight: 110-120 kDa GenBank Accession Number: NM_004444.4 Gene Symbol: EPHB4 Gene ID (NCBI): 2050 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P54760 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.