Proteintech, 85171-3-RR, Cleaved Caspase 8/P18 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cleaved Caspase 8/P18 (85171-3-RR) by Proteintech is a Recombinant antibody targeting Cleaved Caspase 8/P18 in WB, IHC, ELISA applications with reactivity to human samples 85171-3-RR targets Cleaved Caspase 8/P18 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Staurosporine treated Jurkat cells Positive IHC detected in: Jurkat cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). This antibody recognize the cleaved caspase 8. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 8, apoptosis-related cysteine peptidase Calculated Molecular Weight: 538 aa, 62 kDa Observed Molecular Weight: 55 kDa, 18 kDa GenBank Accession Number: BC028223 Gene Symbol: Caspase 8 Gene ID (NCBI): 841 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q14790 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.