Proteintech, 85175-5-RR, Cleaved PARP1 (Asp214) Recombinant monoclonal antibody

Size: 20ul / 100ul The Cleaved PARP1 (Asp214) (85175-5-RR) by Proteintech is a Recombinant antibody targeting Cleaved PARP1 (Asp214) in WB, IF/ICC, ELISA applications with reactivity to human samples 85175-5-RR targets Cleaved PARP1 (Asp214) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Staurosporine treated HeLa cells, Staurosporine treated Jurkat cells Positive IF/ICC detected in: Staurosporine treated Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: poly (ADP-ribose) polymerase 1 Calculated Molecular Weight: 1014 aa, 113 kDa Observed Molecular Weight: 89 kDa GenBank Accession Number: BC037545 Gene Symbol: PARP1 Gene ID (NCBI): 142 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P09874 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.