Proteintech, 85184-5-RR, EPS8 Recombinant monoclonal antibody

Size: 20ul / 100ul The EPS8 (85184-5-RR) by Proteintech is a Recombinant antibody targeting EPS8 in WB, IHC, ELISA applications with reactivity to human, mouse samples 85184-5-RR targets EPS8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, C2C12 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3121 Product name: Recombinant human EPS8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 13-267 aa of BC030010 Sequence: GMYPSQMNGYGSSPTFSQTDREHGSKTSAKALYEQRKNYARDSVSSVSDISQYRVEHLTTFVLDRKDAMITVDDGIRKLKLLDAKGKVWTQDMILQVDDRAVSLIDLESKNELENFPLNTIQHCQAVMHSCSYDSVLALVCKEPTQNKPDLHLFQCDEVKANLISEDIESAISDSKGGKQKRRPDALRMISNADPSIPPPPRAPAPAPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHEQEETPEMMAARID Predict reactive species Full Name: epidermal growth factor receptor pathway substrate 8 Calculated Molecular Weight: 822 aa, 92 kDa Observed Molecular Weight: 97 kDa GenBank Accession Number: BC030010 Gene Symbol: EPS8 Gene ID (NCBI): 2059 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q12929 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.