Proteintech, 85186-3-RR, MAFF Recombinant monoclonal antibody

Size: 20ul / 100ul The MAFF (85186-3-RR) by Proteintech is a Recombinant antibody targeting MAFF in WB, IHC, IF/ICC, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 85186-3-RR targets MAFF in WB, IHC, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, mouse placenta tissue, HuH-7 cells, L02 cells, 4T1 cells Positive IHC detected in: human lung cancer tissue, human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3460 Product name: Recombinant human MAFF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC015037 Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGKCEALQGFARSVAAARGPATLVAPASVITIVKSTPGSGSGPAHGPDPAHGPASCS Predict reactive species Full Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) Calculated Molecular Weight: 17 aa, 8 kDa Observed Molecular Weight: 18-23 kDa GenBank Accession Number: BC015037 Gene Symbol: MAFF Gene ID (NCBI): 23764 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9ULX9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.