Proteintech, 85189-4-RR, GREB1 Recombinant monoclonal antibody

Size: 20ul / 100ul The GREB1 (85189-4-RR) by Proteintech is a Recombinant antibody targeting GREB1 in WB, ELISA applications with reactivity to human samples 85189-4-RR targets GREB1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: T-47D cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag29908 Product name: Recombinant human GREB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1075-1212 aa of NM_014668 Sequence: EVPLEKGARNEALESDAEKLSSTDNEDEELGTEGSTSEKRSPMKRERSRSHDSASSSLSSKASGSALGGESSAQPTALPQGEHARSPQPRGPAEEGRAPGEKQRPRASQGPPSAISRHSPGPTPQPDCSLRTGQRSVQ Predict reactive species Full Name: GREB1 protein Calculated Molecular Weight: 216 kDa Observed Molecular Weight: 216-240 kDa GenBank Accession Number: NM_014668 Gene Symbol: GREB1 Gene ID (NCBI): 9687 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q4ZG55 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.