Proteintech, 85200-1-RR, BAK Recombinant monoclonal antibody

Size: 20ul / 100ul The BAK (85200-1-RR) by Proteintech is a Recombinant antibody targeting BAK in WB, ELISA applications with reactivity to human samples 85200-1-RR targets BAK in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, Jurkat cells, HepG2 cells, THP-1 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information The BAK encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: BCL2-antagonist/killer 1 Calculated Molecular Weight: 23 kDa Observed Molecular Weight: 23-25 kDa GenBank Accession Number: BC004431 Gene Symbol: BAK1 Gene ID (NCBI): 578 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q16611 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.