Proteintech, 85299-5-RR, Nidogen 2 Recombinant monoclonal antibody

Size: 20ul / 100ul The Nidogen 2 (85299-5-RR) by Proteintech is a Recombinant antibody targeting Nidogen 2 in WB, ELISA applications with reactivity to human, mouse samples 85299-5-RR targets Nidogen 2 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Nidogen, also referred to as entactin, represents a highly conserved protein in vertebrates and invertebrates and is found in almost all basement membranes (BM). Nidogen-1 (150 kDa) and nidogen-2 (200 kDa) are elongated molecules composed of three globular domains (G1, G2, and G3) connected by a flexible, protease-sensitive link and a rigid rod-like domain. Western blot analysis of protein extracts of heart, lung, and skeletal muscle of mice, major bands of about 200 kDa were detected, accompanied by smaller and variable bands of 160-170 kDa. (PMID: 12215539) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4643 Product name: Recombinant human NID2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-300 aa of BC035608 Sequence: MEGDRVAGRPVLSSLPVLLLLQLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVPANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITY Predict reactive species Full Name: nidogen 2 (osteonidogen) Calculated Molecular Weight: 1375 aa, 151 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC035608 Gene Symbol: NID2 Gene ID (NCBI): 22795 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q14112 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.