Proteintech, 85363-1-RR, BCL11B Recombinant monoclonal antibody

Size: 20ul / 100ul The BCL11B (85363-1-RR) by Proteintech is a Recombinant antibody targeting BCL11B in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human samples 85363-1-RR targets BCL11B in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, MOLT-4 cells, mouse thymus tissue Positive IF/ICC detected in: Jurkat cells Positive FC (Intra) detected in: Jurkat cells Positive ChIP-qPCR detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: B-cell CLL/lymphoma 11B (zinc finger protein) Calculated Molecular Weight: 96 kDa Observed Molecular Weight: 116 kDa GenBank Accession Number: NM_022898 Gene Symbol: BCL11B Gene ID (NCBI): 64919 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9C0K0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.