Proteintech, 85404-4-RR, RORB Recombinant monoclonal monoclonal antibody

Size: 20ul / 100ul The RORB (85404-4-RR) by Proteintech is a Recombinant antibody targeting RORB in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 85404-4-RR targets RORB in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse retina tissue, rat retina tissue Positive IP detected in: mouse eye tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:15000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology (PMID: 22189870). In addition, NRs that lack identified ligands are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family (PMID: 7935491), which shares a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as the transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone (PMID: 17431400). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11910 Product name: Recombinant human RORB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 106-459 aa of BC051830 Sequence: EQRQQQSGEAEALARVYSSSISNGLSNLNNETSGTYANGHVIDLPKSEGYYNVDSGQPSPDQSGLDMTGIKQIKQEPIYDLTSVPNLFTYSSFNNGQLAPGITMTEIDRIAQNIIKSHLETCQYTMEELHQLAWQTHTYEEIKAYQSKSREALWQQCAIQITHAIQYVVEFAKRITGFMELCQNDQILLLKSGCLEVVLVRMCRAFNPLNNTVLFEGKYGGMQMFKALGSDDLVNEAFDFAKNLCSLQLTEEEIALFSSAVLISPDRAWLIEPRKVQKLQEKIYFALQHVIQKNHLDDETLAKLIAKIPTITAVCNLHGEKLQVFKQSHPEIVNTLFPPLYKELFNPDCATGCK Predict reactive species Full Name: RAR-related orphan receptor B Calculated Molecular Weight: 459 aa, 52 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC051830 Gene Symbol: RORB Gene ID (NCBI): 6096 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q92753 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.