Proteintech, 85449-5-RR, RIOK3 Recombinant monoclonal antibody

Size: 20ul / 100ul The RIOK3 (85449-5-RR) by Proteintech is a Recombinant antibody targeting RIOK3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 85449-5-RR targets RIOK3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, MCF-7 cells, HeLa cells, HEK-293T cells, HepG2 cells Positive IHC detected in: human rectal cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Jurkat cells, A431 cells, MCF-7 cells Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4497 Product name: Recombinant human RIOK3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 215-516 aa of BC039729 Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE Predict reactive species Full Name: RIO kinase 3 (yeast) Calculated Molecular Weight: 516 aa, 60 kDa Observed Molecular Weight: 60-65 kDa GenBank Accession Number: BC039729 Gene Symbol: RIOK3 Gene ID (NCBI): 8780 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14730 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.