Proteintech, 85679-1-RR, Phospho-LCK (Tyr505) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-LCK (Tyr505) (85679-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-LCK (Tyr505) in WB, ELISA applications with reactivity to human samples 85679-1-RR targets Phospho-LCK (Tyr505) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: H2O2 treated Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Lck is comprised of a SH3 domain, binding prolinerich regions, a SH2 domain, binding tyrosine-phosphorylated sequences, a kinase domain, a unique domain, and the negative regulatory tail. The kinase domain of Lck contains 2 important tyrosine residues. Tyr394 and Tyr505 represent the activating and inhibitory tyrosine residue, respectively. Therefore, Lck can exist in 4 distinct states of activity: an inactive state, a primed state, an active state, and a dually phosphorylated active state . Transitions between activity states are governed by phosphorylation at the Tyr394 and Tyr505 residues. When Tyr394 is dephosphorylated while Tyr505 is phosphorylated, Lck is in an inactive state. Lck becomes primed when Tyr505 is dephosphorylated and fully active after Tyr394 phosphorylation. In addition, there is a dually phosphorylated active form of Lck when both Tyr394 and Tyr505 residues are phosphorylated. (PMID: 32794043) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: lymphocyte-specific protein tyrosine kinase Calculated Molecular Weight: 539 aa, 56 kDa Observed Molecular Weight: 56 kDa GenBank Accession Number: BC013200 Gene Symbol: LCK Gene ID (NCBI): 3932 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P06239 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.