Proteintech, 85697-1-RR, RLBP1 Recombinant monoclonal antibody

Size: 20ul / 100ul The RLBP1 (85697-1-RR) by Proteintech is a Recombinant antibody targeting RLBP1 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 85697-1-RR targets RLBP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse eye tissue, ARPE-19 cells, mouse retina tissue, rat retina tissue Positive IHC detected in: mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse eye tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7602 Product name: Recombinant human RLBP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC004199 Sequence: MSEGVGTFRMVPEEEQELRAQLEQLTTKDHGPVFGPCSQLPRHTLQKAKDELNEREETREEAVRELQEMVQAQAASGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNFRLQYPELFDSLSPEAVRCTIEAGYPGVLSSRDKYGRVVMLFNIENWQSQEITFDEILQAYCFILEKLLENEETQINGFCIIENFKGFTMQQAASLRTSDLRKMVDMLQDSFPARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEIDENILPSDFGGTLPKYDGKAVAEQLFGPQAQAENTAF Predict reactive species Full Name: retinaldehyde binding protein 1 Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC004199 Gene Symbol: RLBP1 Gene ID (NCBI): 6017 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P12271 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.