Proteintech, 85709-1-RR, DDX10 Recombinant monoclonal antibody

Size: 20ul / 100ul The DDX10 (85709-1-RR) by Proteintech is a Recombinant antibody targeting DDX10 in WB, ELISA applications with reactivity to human samples 85709-1-RR targets DDX10 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SGC-7901 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag20766 Product name: Recombinant human DDX10 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 100-431 aa of BC091521 Sequence: IGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETVSFHATDLQMLVLDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLARLSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLL Predict reactive species Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 Calculated Molecular Weight: 875 aa, 101 kDa Observed Molecular Weight: 101-110 kDa GenBank Accession Number: BC091521 Gene Symbol: DDX10 Gene ID (NCBI): 1662 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13206 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.