Proteintech, 85725-1-RR, ALDH3A1 Recombinant monoclonal antibody

Size: 20ul / 100ul The ALDH3A1 (85725-1-RR) by Proteintech is a Recombinant antibody targeting ALDH3A1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 85725-1-RR targets ALDH3A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, BxPC-3 cells, MG-63 cells, mouse stomach tissue Positive IHC detected in: mouse liver tissue, mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7977 Product name: Recombinant human ALDH3A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 105-453 aa of BC004370 Sequence: LGVVLVIGTWNYPFNLTIQPMVGAIAAGNAVVLKPSELSENMASLLATIIPQYLDKDLYPVINGGVPETTELLKERFDHILYTGSTGVGKIIMTAAAKHLTPVTLELGGKSPCYVDKNCDLDVACRRIAWGKFMNSGQTCVAPDYILCDPSIQNQIVEKLKKSLKEFYGEDAKKSRDYGRIISARHFQRVMGLIEGQKVAYGGTGDAATRYIAPTILTDVDPQSPVMQEEIFGPVLPIVCVRSLEEAIQFINQREKPLALYMFSSNDKVIKKMIAETSSGGVAANDVIVHITLHSLPFGGVGNSGMGSYHGKKSFETFSHRRSCLVRPLMNDEGLKVRYPPSPAKMTQH Predict reactive species Full Name: aldehyde dehydrogenase 3 family, memberA1 Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 48-52 kDa GenBank Accession Number: BC004370 Gene Symbol: ALDH3A1 Gene ID (NCBI): 218 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P30838 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.