Proteintech, 85730-1-RR, NCDN Recombinant monoclonal antibody

Size: 20ul / 100ul The NCDN (85730-1-RR) by Proteintech is a Recombinant antibody targeting NCDN in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 85730-1-RR targets NCDN in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue, fetal human brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information NCDN protein (Neurochondrin) is a highly conserved 79 kDa adaptor protein, which was first discovered in 1997 and mainly expressed in the nervous system. NCDN protein plays an important role in neuronal differentiation, formation and maintenance of dendritic spines. It is involved in the development of synapses, neuronal differentiation and signal transduction, and maintains cell polarity by regulating dendritic morphogenesis and neural signal pathway. Recent studies have found that NCDN protein is expressed not only in neurons, but also in myeloid cells (such as neutrophils), and plays a role as a negative regulator of innate immunity. Mice lacking NCDN in myeloid cells are more immune to bacterial infection because of the increased reactivity of neutrophils (PMID: 37503670). The molecular weight of NCDN is 78 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3859 Product name: Recombinant human NCDN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 426-729 aa of BC024592 Sequence: KTLYEEAEEANDLSQQVANLAISPTTPGPTWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWELTSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLMTSLPALVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAILFLSQSHVARATPGSDQAVLALSPEYEGIWADLQELWFLGMQAFTGCVPLLPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRLCREAMRLQAGEETASHYRMAALEQCLSEP Predict reactive species Full Name: neurochondrin Calculated Molecular Weight: 729 aa, 79 kDa Observed Molecular Weight: 75-79 kDa GenBank Accession Number: BC024592 Gene Symbol: Neurochondrin Gene ID (NCBI): 23154 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UBB6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.