Proteintech, 85734-1-RR, DNALI1 Recombinant monoclonal antibody

Size: 20ul / 100ul The DNALI1 (85734-1-RR) by Proteintech is a Recombinant antibody targeting DNALI1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 85734-1-RR targets DNALI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, rat testis tissue Positive IHC detected in: mouse fallopian tube tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag11652 Product name: Recombinant human DNALI1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-258 aa of BC046117 Sequence: MIPPADSLLKYDTPVLVSRNTEKRSPKARLLKVSPQQPGPSGSAPQPPKTKLPSTPCVPDPTKQAEEILNAILPPREWVEDTQLWIQQVSSTPSTRMDVVHLQEQLDLKLQQRQARETGICPVRRELYSQCFDELIREVTINCAERGLLLLRVRDEIRMTIAAYQTLYESSVAFGMRKALQAEQGKSDMERKIAELETEKRDLERQVNEQKAKCEATEKRESERRQVEEKKHNEEIQFLKRTNQQLKAQLEGIIAPKK Predict reactive species Full Name: dynein, axonemal, light intermediate chain 1 Calculated Molecular Weight: 258 aa, 30 kDa Observed Molecular Weight: 30-33 kDa GenBank Accession Number: BC046117 Gene Symbol: DNALI1 Gene ID (NCBI): 7802 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14645 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.