Proteintech, 85788-5-RR, CDC5L Recombinant monoclonal antibody

Size: 20ul / 100ul The CDC5L (85788-5-RR) by Proteintech is a Recombinant antibody targeting CDC5L in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 85788-5-RR targets CDC5L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, U2OS cells, HCT 116 cells, NIH/3T3 cells, Neuro-2a cells Positive IHC detected in: human spleen tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:8000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information CDC5L is ubiquitously expressed in humans, with relatively high expression levels in testis, bone marrow, and 25 other tissues. In proliferating cells, CDC5L is constitutively expressed, suggesting its regulation may occur through post-translational modifications. CDC5L plays a critical role in the cell cycle, particularly in the G2/M transition. It interacts with cyclins and cyclin-dependent kinases (CDKs), helping to control the timing and accuracy of cell division. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3578 Product name: Recombinant human CDC5L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 451-803 aa of BC001568 Sequence: NPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF Predict reactive species Full Name: CDC5 cell division cycle 5-like (S. pombe) Calculated Molecular Weight: 802 aa, 92 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC001568 Gene Symbol: CDC5L Gene ID (NCBI): 988 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99459 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.