Proteintech, 85804-1-RR, Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Myosin Light Chain 2 (Thr18/Ser19) (85804-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Myosin Light Chain 2 (Thr18/Ser19) in WB, ELISA applications with reactivity to human, mouse, rat samples 85804-1-RR targets Phospho-Myosin Light Chain 2 (Thr18/Ser19) in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Calyculin A treated NIH/3T3 cells, Calyculin A treated HEK-293 cells, Calyculin A treated HSC-T6 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myosin, light chain 12A, regulatory, non-sarcomeric Calculated Molecular Weight: 171 aa, 20 kDa Observed Molecular Weight: 19-20 kDa GenBank Accession Number: BC016372 Gene Symbol: MYL12A Gene ID (NCBI): 10627 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P19105 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.