Proteintech, 85811-2-RR, DOPA decarboxylase/DDC Recombinant monoclonal antibody

Size: 20ul / 100ul The DOPA decarboxylase/DDC (85811-2-RR) by Proteintech is a Recombinant antibody targeting DOPA decarboxylase/DDC in WB, ELISA applications with reactivity to human, mouse samples 85811-2-RR targets DOPA decarboxylase/DDC in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SH-SY5Y cells, mouse brain tissue, mouse kidney tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information DOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily. DDC is an enzyme that converts levodopa into dopamine14, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra (PMID: 3374198, PMID: 28100251). DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0219 Product name: Recombinant human DDC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 121-389 aa of BC008366 Sequence: WLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMVATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVQLSHEFE Predict reactive species Full Name: dopa decarboxylase (aromatic L-amino acid decarboxylase) Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 48-50 kDa GenBank Accession Number: BC008366 Gene Symbol: DOPA decarboxylase Gene ID (NCBI): 1644 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P20711 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.