Proteintech, 85845-1-RR, Nephrin Recombinant monoclonal antibody

Size: 20ul / 100ul The Nephrin (85845-1-RR) by Proteintech is a Recombinant antibody targeting Nephrin in WB, ELISA applications with reactivity to human, mouse samples 85845-1-RR targets Nephrin in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag18994 Product name: Recombinant human NPHS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 89-374 aa of BC156935 Sequence: PARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRW Predict reactive species Full Name: nephrosis 1, congenital, Finnish type (nephrin) Calculated Molecular Weight: 1241 aa, 135 kDa Observed Molecular Weight: 120-130 kDa GenBank Accession Number: BC156935 Gene Symbol: Nephrin Gene ID (NCBI): 4868 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O60500 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.