Proteintech, 85855-1-RR, DNA-RNA Hybrid Recombinant monoclonal antibody

Size: 20ul / 100ul The DNA-RNA Hybrid (85855-1-RR) by Proteintech is a Recombinant antibody targeting DNA-RNA Hybrid in IHC, IF/ICC, FC (Intra), Dot blot, ELISA applications with reactivity to n/a samples 85855-1-RR targets DNA-RNA Hybrid in IHC, IF/ICC, FC (Intra), Dot blot, ELISA applications and shows reactivity with n/a samples. Tested Applications Positive IHC detected in: human placenta tissue, human ovary tumor tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Positive FC (Intra) detected in: HEK-293T cells Positive Dot blot detected in: / / Recommended dilution Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension DOT BLOT: DOT BLOT : 1:10-1:100 Background Information DNA-RNA Hybrid antibodies are primarily used to study R-loops. Theoretically, they do not bind to single-stranded DNA, double-stranded DNA, or RNA. R-loops are DNA-RNA hybrid structures formed during transcription and are closely related to genome stability, DNA replication, DNA repair, and cancer development. Specification Tested Reactivity: n/a Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: unknow Predict reactive species Full Name: DNA-RNA Hybrid Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.