Proteintech, 85875-1-RR, Hexokinase 2 Recombinant monoclonal antibody

Size: 20ul / 100ul The Hexokinase 2 (85875-1-RR) by Proteintech is a Recombinant antibody targeting Hexokinase 2 in WB, IHC, ELISA applications with reactivity to human, mouse samples 85875-1-RR targets Hexokinase 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, mouse heart tissue, Jurkat cells, MCF-7 cells, SH-SY5Y cells, HCT 116 cells, mouse skeletal muscle tissue Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag16895 Product name: Recombinant human HK2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 568-917 aa of BC021116 Sequence: HGTGDELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCQQNSLDESILLKWTKGFKASGCEGEDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGFEDPHCEVGLIVGTGSNACYMEEMRNVELVEGEEGRMCVNMEWGAFGDNGCLDDFRTEFDVAVDELSLNPGKQRFEKMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALLQVRAILQHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGMAAVVDRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLQSEDGSGKGAALITAVACRIREAGQR Predict reactive species Full Name: hexokinase 2 Calculated Molecular Weight: 102 kDa Observed Molecular Weight: 102-120 kDa GenBank Accession Number: BC021116 Gene Symbol: Hexokinase 2 Gene ID (NCBI): 3099 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P52789 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.