Proteintech, 85930-1-RR, FOXG1 Recombinant monoclonal antibody

Size: 20ul / 100ul The FOXG1 (85930-1-RR) by Proteintech is a Recombinant antibody targeting FOXG1 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 85930-1-RR targets FOXG1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IF/ICC detected in: C6 cells, U-251 cells Positive FC (Intra) detected in: U251 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension Background Information Forkhead Box G1 (FOXG1) is a member of the Forkhead family of genes with non-redundant roles in brain development, where alteration of this gene's expression significantly affects the formation and function of the mammalian cerebral cortex. FOXG1 haploinsufficiency in humans is associated with prominent differences in brain size and impaired intellectual development noticeable in early childhood, while homozygous mutations are typically fatal. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag17169 Product name: Recombinant human FOXG1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 190-489 aa of BC035020 Sequence: IMMAIRQSPEKRLTLNGIYEFIMKNFPYYRENKQGWQNSIRHNLSLNKCFVKVPRHYDDPGKGNYWMLDPSSDDVFIGGTTGKLRRRSTTSRAKLAFKRGARLTSTGLTFMDRAGSLYWPMSPFLSLHHPRASSTLSYNGTTSAYPSHPMPYSSVLTQNSLGNNHSFSTANGLSVDRLVNGEIPYATHHLTAAALAASVPCGLSVPCSGTYSLNPCSVNLLAGQTSYFFPHVPHPSMTSQSSTSMSARAASSSTSPQAPSTLPCESLRPSLPSFTTGLSGGLSDYFTHQNQGSSSNPLIH Predict reactive species Full Name: forkhead box G1 Calculated Molecular Weight: 489 aa, 52 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC035020 Gene Symbol: FOXG1 Gene ID (NCBI): 2290 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P55316 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.