Proteintech, 86139-2-RR, Collagen Type II Recombinant monoclonal antibody

Size: 20ul / 100ul The Collagen Type II (86139-2-RR) by Proteintech is a Recombinant antibody targeting Collagen Type II in WB, IHC, ELISA applications with reactivity to human samples 86139-2-RR targets Collagen Type II in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: TT cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag29265 Product name: Recombinant human COL2A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC007252 Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART Predict reactive species Full Name: collagen, type II, alpha 1 Calculated Molecular Weight: 134 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC007252 Gene Symbol: COL2A1 Gene ID (NCBI): 1280 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P02458 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.