Proteintech, 86195-4-RR, Frataxin Recombinant monoclonal antibody

Size: 20ul / 100ul The Frataxin (86195-4-RR) by Proteintech is a Recombinant antibody targeting Frataxin in WB, ELISA applications with reactivity to human samples 86195-4-RR targets Frataxin in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SW480 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Frataxin (FXN), also named as FRDA, X25, m81-FXN, d-FXN, m78-FXN and i-FXN, belongs to the frataxin family. It promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. FXN may play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. FXN is cleaved to be 4 chains. FXN is expressed in the cytoplasm as a 210 amino acid (AA) precursor protein (pFXN; 23 KDa) that is translocated into mitochondria where it is processed by two consecutive steps into iFXN (FXN 42-210; 19 KDa) and finally mFXN (81-210; 14.2 KDa), which is functional. (PMID: 26704351, PMID: 26671574) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg4622 Product name: Recombinant Human Frataxin protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 42-210 aa of NM_000144.5 Sequence: LRTDIDATCTPRRASSNQRGLNQIWNVKKQSVYLMNLRKSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELLAAELTKALKTKLDLSSLAYSGKDA Predict reactive species Full Name: frataxin Calculated Molecular Weight: 23kDa Observed Molecular Weight: 14-23 kDa GenBank Accession Number: NM_000144.5 Gene Symbol: FXN Gene ID (NCBI): 2395 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16595-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.